Dr .Prakash Chandra Tiwari Poonam Pandey


Pentachlorophenol (PCP) is a toxic chlorinated hydrocarbon due its biocidal properties mainly used as a preservative in leather industries. With a new enrichment protocol, fifteen bacterial strains were isolated from sediment sludge of tannery effluent (Unnao, Kanpur, U.P. India). These were screened out for their PCP degrading potential by using minimal salt agar medium containing, sodium salt of pentachlorophenol (NaPCP) as sole source of carbon and energy and bromothymol blue as screening agent. Only nine strains were found capable of degrading PCP and these were characterized morphologically and biochemically and were shared characteristics of the genus as Bacillus species (three strains), Flavobacterium species (two strains) Pseudomonas species (two strains) and Proteus species (two strains). The PCP degrading potential of each individual species was assessed in lab scale bioreactor. It was observed that Flavobacterium species has highest PCP degrading potential as it degraded 90% PCP within 24 hours followed by Pseudomonas species and Bacillus species (88% and 69% respectively). The Proteus species showed lowest degradation potential (54%) within the same time. Further, combination of different strains were designed for the assessment of consortia based comparative degradation of PCP in which combination of Flavobacterium species with Pseudomonas species showed significant reduction of 90% in PCP concentration as compared to other possible combinations.

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